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KPT185

SKU: orb1305500

Description

KPT-185 is a potent and selective inhibitor of CRM1 (exportin 1). It induces cell-cycle arrest and apoptosis, making it a valuable tool for studying nuclear export in cancer research, both in vitro and in vivo models.

Research Area

Pharmacology & Drug Discovery

Images & Validation

Key Properties

CAS Number1333151-73-7
MW355.31
Purity99.31% (May vary between batches)
FormulaC16H16F3N3O3
SMILESCOc1cc(cc(c1)C(F)(F)F)-c1ncn(C=CC(=O)OC(C)C)n1
TargetCRM1
SolubilityEthanol:66 mg/mL (185.75 mM);DMSO:66 mg/mL (185.75 mM);H2O:<1 mg/mL

Bioactivity

Target IC50
CRM1:100-500nM
In Vivo
In leukemia cells (IC50=100 nM-500 nM), KPT-185 significantly inhibited cell proliferation. In AML cell lines and primary AML mother cells, KPT-185 was able to arrest the cell cycle and induce apoptosis. In pancreatic cancer cells, KPT-185 inhibited cell proliferation and induced apoptosis, but KPT-185 did not affect the growth of human pancreatic ductal epithelial cells.
Cell Research
Cells are seeded into 96-well plates and treated for 24, 48, and 72 hours with KPT-SINE at various concentrations ranging from 10 nM to 10 μM. Cell viability is evaluated using the cell proliferation reagent WST-1 according to the manufacturer's protocol. The absorbance of wells at 450 nm (reference wavelength, 650 nm) is measured with a microplate reader. (Only for Reference)

Storage & Handling

Storagestore at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

KPT 185, KPT185, KPT-185, CRM1
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Key Properties

No computed properties available.

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Protocol Information

KPT185 (orb1305500)

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2 mg
¥ 910.00
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