Cytokeratin 19 Antibody

SKU: orb2638788

Description

Recognizes a protein of 40kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.

Images & Validation

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Tested Applications	IHC-P
Dilution Range	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Reactivity	Human
Application Notes	Optimal dilution of the Cytokeratin 19 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. Read more...

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2a, lambda
Clone No.	KRT19/799
Immunogen	Recombinant human KRT19 protein was used as the immunogen for the Cytokeratin 19 antibody.
Purification	Protein G affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Cytokeratin 19 Rabbit Polyclonal Antibody [orb5872]

Applications:	FC, IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Equine, Gallus, Mouse, Porcine, Rabbit, Rat
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Sizes Available: 50 μl, 200 μl, 100 μl

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Cytokeratin Antibody Panel [orb3130872]
Applications:
IF, IHC, WB
Reactivity:
Human, Mouse, Rat
Conjugation:
Unconjugated
Sizes Available: 1 kit (5 x 25 μl)

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IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human cervical carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human endometrial carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Cytokeratin 19 antibody (KRT19/799).

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with Cytokeratin 19 antibody (KRT19/799).

SDS-PAGE analysis of purified, BSA-free Cytokeratin 19 antibody (clone KRT19/799) as confirmation of integrity and purity.

Quick Database Links

UniProt

P08727

UniProt Details

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No UniProt data available

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Protocol Information

IHC-P

Immunohistochemistry Paraffin

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