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KRT8 Antibody

SKU: orb2637815

Description

KRT8, also called Cytokeratin 8, is a low molecular weight keratin of approximately 50 kDa. It participates in cellular differentiation and signal transduction, protects against apoptosis, stress and injury, and helps maintain cellular structural integrity. KRT8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas.

Images & Validation

Tested ApplicationsIHC-P
Dilution RangeThe prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
ReactivityHuman
Application Notes
Differences in protocols and secondaries may require the KRT8 antibody to be titered for optimal performance.1. FFPE staining requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.K8.8
ImmunogenHuman carcinoma keratin was used as the immunogen for this KRT8 antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPrediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

KRT8 Antibody

IHC testing of human colon carcinoma stained with KRT8 antibody (K8.8).

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Protocol Information

IHC-P
Immunohistochemistry Paraffin
View Protocol

KRT8 Antibody (orb2637815)

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7 ml
¥ 9,750.00
Free Secondary Antibody (20 ul)0/0

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