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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−Item 1 of 1
| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, IP=1:20-50, Flow-Cyt=1:50-100 |
| Reactivity | Human |
| Predicted Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Isotype | IgG |
| Clone No. | B3E6 |
| Immunogen | A synthesized peptide derived from human Ku70 (525-568aa) |
| Target | XRCC6 |
| Molecular Weight | 68 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% rAlbumin, 0.02% Proclin300 and 50% glycerol. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CTC75; CTCBF; G22P1; KU70; ML8; TLAA; 70kDa; XRCC6_HUMAN; XRCC6; 5'-deoxyribose-5-phosphate lyase Ku70 (5'-dRP lyase Ku70); 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit (CTC75 | CTCBF); DNA repair protein XRCC6; Lupus Ku autoantigen protein p70 (Ku70); Thyroid-lupus autoantigen (TLAA); X-ray repair complementing defective repair in Chinese hamster cells 6; 3.6.4.-; 4.2.99.-; XRCC6_MOUSE; 5'-deoxyribose-5-phosphate lyase Ku70 (5'-dRP/AP lyase Ku70); Ku autoantigen protein p70 homolog (Ku70);

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Western blot analysis of HeLa cell lysate. Using Ku70 / XRCC6 (orb2562651) monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Quick Database Links
Gene Symbol
XRCC6
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
Ku70 / XRCC6 Recombinant Rabbit Monoclonal Antibody (orb2562651)
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