L-161982

SKU: orb1308147

Description

L-161982 is a selective EP4 prostaglandin receptor antagonist. It suppresses PGE2-mediated ERK phosphorylation and proliferation in HCA-7 colon carcinoma cells in vitro and demonstrates efficacy in a murine collagen-induced arthritis model, supporting research in oncology and inflammation.

Research Area

Immunology & Inflammation, Pharmacology & Drug Discovery

Images & Validation

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Key Properties

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CAS Number	147776-06-5
MW	654.72
Purity	99.91%
Formula	C₃₂H₂₉F₃N₄O₄S₂
SMILES	O=C1N(N=C(CCCC)N1CC2=CC=C(C=C2)C3=C(S(NC(=O)C4=C(C)C=CS4)(=O)=O)C=CC=C3)C5=C(C(F)(F)F)C=CC=C5
Target	Prostaglandin Receptor
Solubility	DMSO:9 mg/mL (13.75 mM);10% DMSO+90% Corn Oil:0.5 mg/mL (0.76 mM)

Bioactivity

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In Vivo

METHODS: The CIA mouse model was treated with L161982 (5 mg/kg, intraperitoneal injection, daily, 2 weeks) to evaluate the total arthritis score and histopathological examination to determine the severity of CIA; the plasma and tissue expressions of IL-17 and monocyte chemoattractant protein-1 (MCP-1) were detected by enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining (IHC), respectively; the proliferation of isolated Tregs and the Th17 polarization rate of naive T cells under L161982 treatment were tested by BrdU assay and flow cytometry, respectively. RESULTS CIA mice treated with L161982 showed reduced arthritis scores, joint swelling, cracking of cartilage surface, and decreased hyperplasia in the connective tissue of the joint cavity. Plasma and tissue IL-17 and MCP-1 were reduced, while the proportion of Treg cells in the spleen and lymph nodes of CIA mice was increased. Otherwise, L161982 had no direct effect on the proliferation of Tregs; a reduced trend toward Th17 polarization in vitro was observed in L161982-treated naive T cells.

In Vitro

METHODS: HCA-7 cells were pre-incubated with L-161,982 (10 μM) and then stimulated with PGE for 72 h to evaluate whether inhibition of EP4 receptors by L-161,982 inhibits cell proliferation of PGE-stimulated HCA-7 cells. RESULTS Pre-treatment with L-161,982 blocked PGE2-induced cell proliferation. METHODS: HCA-7 cells were pretreated with L-161982 (10 μM, 1 hour) and then stimulated with PGE2 for another 1 hour to investigate whether L-161982 would lead to inhibition of ERK activation as assessed by ERK phosphorylation status. RESULTS ERK phosphorylation was completely blocked in HCA-7 cells pretreated with L-161982, indicating that L-161982 mediates PGE2-induced ERK phosphorylation in HCA-7 cells. METHODS: Tca8113 cells were stimulated with L-161982 (5, 10, 15, 20μM) for 24, 48 and 72 hours to study the effect of L-161982 on Tca8113 cell proliferation. RESULTS The proliferation of Tca8113 cells was significantly reduced in a dose- and time-dependent manner; after 48 hours, IC50 was obtained at 15 μM; L-161982 inhibited the cell proliferation of Tca8113 cells in a dose- and time-dependent manner.

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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colon, arthritis, cancer, inhibit, L 161982, EP4, Inhibitor, L161982, L-161982, phosphorylation, proliferation, prostaglandin, ProstaglandinReceptor, Prostaglandin Receptor

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Compound Identifiers

PubChem CID

9961192 ↗

Key Properties

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No computed properties available.

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L-161982

Description

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Key Properties

Bioactivity

Storage & Handling

Alternative Names

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Compound Identifiers

Key Properties

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L-161982 (orb1308147)

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