Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Latency-associated peptide (101-200/390aa)
Target	TGFB1
Molecular Weight	30/44 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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LAP; Latency-associated peptide; ARVD; BSC 1 cell growth inhibitor; CED; Cetermin; Diaphyseal dysplasia 1 progressive; DPD 1; DPD1; G TSF; Glioblastoma derived T cell suppressor factor; LAP; Polyergin; TGF beta 1; TGF beta 1 protein; TGF beta; TGF beta1; TGF-beta-1; TGFB 1; TGFB; TGFB1; TGFB1_HUMAN; TGFbeta; Transforming growth factor; Transforming growth factor beta 1; Transforming Growth Factor beta.

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Latency-associated peptide Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (TGFB1) Polyclonal Antibody, Unconjugated (orb2885) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Latency-associated peptide) Polyclonal Antibody, Unconjugated (orb2885) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: K562 (Human) Cell Lysate at 30 ug, Lane 3: HL60 (Human) Cell Lysate at 30 ug, Primary: Anti-Latency-associated peptide (orb2885) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50/35 kD, Observed band size: 50/33 kD.

Quick Database Links

Gene Symbol

TGFB1

UniProt

P01137

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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