LEU5 Rabbit Polyclonal Antibody

SKU: orb101001

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Description

LEU5 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

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Item 1 of 2

Tested Applications	WB
Dilution Range	WB=1:500-2000
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Canine, Equine, Porcine, Rabbit, Sheep

Related Conjugates & Formulations

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Biotin

HRP

IRDye800

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human LEU5/RFP2 (151-250/407aa)
Target	TRIM13
Molecular Weight	47 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CAR; DLEU5; LEU5; RFP2; RNF77; TRI13_HUMAN; TRIM13; B-cell chronic lymphocytic leukemia tumor suppressor Leu5; Leukemia-associated protein 5; Putative tumor suppressor RFP2; RING finger protein 77; RING-type E3 ubiquitin transferase TRIM13; Ret finger protein 2; Tripartite motif-containing protein 13; 2.3.2.27;

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Sample: Lane 1: Mouse Testis Lysates, Lane 2: Rat Testis Lysates, Lane 3: Rat Lymph Lysates, Lane 4: Human HL-60 cell Lysates, Lane 5: Human SH-SY5Y cell Lysates, Lane 6: Human HeLa cell Lysates, Primary: Anti-LEU5 (orb101001) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47kDa, Observed band size: 47kDa.

Sample: Liver (Mouse) Lysate at 40 ug, Primary: Anti-LEU5 (orb101001) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48 kD.

Quick Database Links

Gene Symbol

TRIM13

UniProt

O60858

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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