Lithocholic acid

SKU: orb1306119

Description

Lithocholic acid

Research Area

Cell Biology, Metabolism Research

Images & Validation

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Key Properties

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CAS Number	434-13-9
MW	376.57
Purity	98.00% (May vary between batches)
Formula	C₂₄H₄₀O₃
SMILES	[H][C@@]12CC[C@H]([C@H](C)CCC(O)=O)[C@@]1(C)CC[C@@]1([H])[C@@]2([H])CC[C@]2([H])C[C@H](O)CC[C@]12C
Target	Apoptosis,Autophagy,Endogenous Metabolite,FXR
Solubility	H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:44 mg/mL (116.84 mM);DMSO:49 mg/mL (130.12 mM)

Bioactivity

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Target IC50	DU-145 cells:30.4 µM\|HCT116 cells (p53−/−):37.4 ± 3.8 μM (LD50)\|PC3 cells:32.0 µM\|HCT116 cells (wt):46.8 ± 2.6 μM (LD50)\|USP2a:31.1 ± 3.4 µM
In Vivo	METHODS: To investigate the antiviral effect of Lithocholic acid, Lithocholic acid oleate (3%) and Lithocholic acid (0.5%), derivatives of LCA, were administered to HSV-1 virus infected mice. RESULTS: Mice treated with Lithocholic acid oleate+Lithocholic acid 48 hours before virus inoculation showed lesions on day 6 after infection, and the wound healing was better than that of the acyclovir treatment group, with reduced scar formation and better skin repair. Read more...
In Vitro	METHODS: Human A549, HCT-116, HT-29, Huh-7, LoVo, MGC-803, RKO, SK-HEP1, SW480 cells were treated with Lithocholic acid (200, 100, 50, P < 0.05). 25, 12.5, 6.25, 3.125, 1.5625 μM) were used to detect the cell growth inhibition by MTT method. RESULTS: Lithocholic acid did not affect the growth of A549, HCT-116, HT-29, Huh-7, LoVo, MGC-803, RKO, SK-HEP1, and SW480 cells (IC50> 200 µM). METHODS: Human Caco2 and HT-1080 cells were treated with Lithocholic acid (0.1-1000 μM) for 24 hours, and the cytotoxicity was detected by MTT assay. RESULTS: Lithocholic acid significantly inhibited the growth of Caco2 (IC50=56 μM) and HT-1080 cells (IC50=23 μM). METHODS: Human DLD1, HCT-116, and HCT-8 cells were treated with Lithocholic acid (25, 50, 100, 150, and 200μM) for 48 hours, and the cytotoxicity was detected by MTT assay. RESULTS: Lithocholic acid significantly inhibited the growth of human DLD1 (IC50=173.1 μM), HCT-116 (IC50=81.1 μM) and HCT-8 (IC50=97.4 μM) cells. Read more...

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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VD, PXR, 3alpha-Hydroxy-5beta-cholanic acid, 3α-Hydroxy-5β-cholanic acid, 3a-Hydroxy-5b-cholanic acid, Autophagy, Apoptosis, Lithocholic acid, inhibit, Inhibitor, Endogenous Metabolite, EndogenousMetabolite, FXR

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Compound Identifiers

PubChem CID

9903 ↗

Key Properties

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No computed properties available.

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