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M Mlkl Antibody (C-term)
SKU: orb1935968
Description
Research Area
Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | IHC-P: 1~400, WB: 1:2000, WB: 1:2000, WB: 1:1000, WB: 1:2000 |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB34842 |
| Target | This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl. |
| Molecular Weight | 54317 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818}
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Western blot analysis of lysate from mouse lung tissue lysate, using Mlkl Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.
Immunohistochemical analysis of paraffin-embedded Human tonsil section using M Mlkl antibody. Diluted at 1~400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
All lanes: Anti-M Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 2: mouse spleen lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 53KDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: mouse testis lysates. Lane 2: mouse liver lysates. Lane 3: NIH/3T3 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Mlkl Antibody (C-term) at 1:2000 dilution. Lane 1: mouse lung lysates. Lane 2: NIH/3T3 whole cell lysates. Lane 3: mouse liver lysates. Lane 4: mouse testis whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 54 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Quick Database Links
Gene Symbol
This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
UniProt
RefSeq (Protein):NP_083281.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_083281.1 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
M Mlkl Antibody (C-term) (orb1935968)
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