MAG Antibody (Center)

SKU: orb1928248

Description

Research Area

Neuroscience

Images & Validation

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Item 1 of 5

Tested Applications	FC, WB
Dilution range	WB - 1:2000
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	69069 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Alternative Names

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GMA

Quality Guarantee

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MAG Antibody (Center) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-MAG Antibody (Center) at 1:2000 dilution + Rat brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-MAG Antibody (Center) at 1:2000 dilution + Human brain whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MAG Antibody (Center) at 1:2000 dilution. Lane 1: Human brain whole tissue lysate. Lane 2: Human cerebellum whole tissue lysate. Lane 3: Mouse brain whole tissue lysate. Lane 4: Mouse cerebellum whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 69 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

UniProt

P20916

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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