MAP3K5 Antibody(T842)

SKU: orb1928771

Description

Research Area

Cardiovascular Research, Cell Biology, Immunology & Inflammation, Infectious Disease & Virology, Neuroscience

Images & Validation

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Item 1 of 3

Tested Applications	IHC-P, WB
Dilution Range	WB - 1:1000, IHC-P - 1:100-500
Reactivity	Human
Predicted Reactivity	Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	154537 Da
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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ASK1, MAPKKK5, MEKK5

Quality Guarantee

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Western blot analysis of MAP3K5 (arrow) using rabbit polyclonal MAP3K5 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2).

All lanes: Anti-MAP3K5 Antibody (T842) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Raji whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 155 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP3K5 ANtibody (T842), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

UniProt

Q99683

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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