Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human Matriptase (11-100/855aa)
Target	TMPRSS6
Molecular Weight	94 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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transmembrane protease, serine 6; 1300008A22Rik; IRIDA; MATRIPTASE2; TMPRSS6.

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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Matriptase) polyclonal Antibody, Unconjugated (orb11026) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Jurkat (Human) CellLysate at 40 ug, Primary: Anti-Matriptase (orb11026) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 94 kD, Observed band size: 125 kD.

Quick Database Links

Gene Symbol

TMPRSS6

UniProt

Q8IU80

UniProt Details

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No UniProt data available

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Protocol Information

ICC

Immunocytochemistry

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