mCherry Antibody

SKU: orb1463300

Description

Goat polyclonal antibody to mCherry (Cherry fluorescent protein). mCherry protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. mCherry is a ~27 kDa protein that is optimally excited at a 587 nm and has a maximum of emission at 610 nm. It is a basic (constitutively fluorescent) red fluorescent protein and It is reported to be a very rapidly-maturing monomer with low acid sensitivity.

Images & Validation

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Tested Applications	IEM, IF, IHC-Fr, IHC-P, WB
Dilution range	WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Application Notes	The antibody solution should be gently mixed before use.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Antigen: Purified recombinant peptide produced in E. coli.. Antigen Sequence: MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK
Target	Red Fluorescent Protein
Purification	Epitope affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	NaHCO3 100 mM pH 9; no preservatives
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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red fluorescent protein antibody.

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Quick Database Links

Gene Symbol

Red Fluorescent Protein

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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