MDM2 Antibody (APC)

SKU: orb535758

Description

Rabbit polyclonal antibody against MDM2 conjugated to APC

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1:1000); IHC (1:50)
Reactivity	Human, Mouse, Rat
Application Notes	#REF!

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	Synthetic peptide from the mid-protein of Human MDM2 (aa. 100-200)
Target	MDM2
Molecular Weight	50 kDa
Purification	Peptide Affinity Purified
Conjugation	APC

Storage & Handling

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Storage	Conjugated antibodies should be stored according to the product label
Buffer/Preservatives	95.46mM Phosphate, 2.48mM MES and 2mM EDTA
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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MDM2, Double minute 2 protein, Hdm2, MTBP, Mouse Double Minute 2, HDMX, ACTFS, p53 Binding Protein Mdm2, Ubiquitin protein ligase E3 Mdm2, Mdm2 p53 E3 ubiquitin protein ligase homolog, MDM2BP, p53-binding protein Mdm2, Oncoprotein Mdm2, Hdm 2, E3 ubiquitin-protein ligase Mdm2, MDM 2, EC:6.3.2.-, MDM2_HUMAN, MDM2 oncogene E3 ubiquitin protein ligase, Murine Double Minute Chromosome 2, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa

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Immunohistochemistry analysis using Rabbit Anti-MDM2 Polyclonal Antibody. Tissue: Stomach Cancer. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.

Western blot analysis of Mouse, Rat Brain showing detection of ~40 kDa MDM2 protein using Rabbit Anti-MDM2 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Lane 3: Rat Brain. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-MDM2 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~40 kDa. Other Band (s): Isoform 2 or 3.

Quick Database Links

Gene Symbol

MDM2

UniProt

Q00987

NCBI Gene ID

4193

RefSeq (Protein):NP_001138811.1

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001138811.1

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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