MEK2 (MAP2K2) Antibody (Center)

The anti-MAP2K2 Pab is used in Western blot to detect MAP2K2 in mouse liver tissue lysate.

MAP2K2 Antibody (A278) western blot analysis in U937 cell line lysates (35 ug/lane).This demonstrates the MAP2K2 antibody detected the MAP2K2 protein (arrow).

Western blot analysis of MAP2K2 (arrow) using MAP2K2 Antibody (Center).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Fluorescent image of U251 cell stained with MEK2 (MAP2K2) Antibody (Center).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEK2 primary antibody (1: 25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). MEK2 immunoreactivity is localized to Cytoplasm significantly.