METTL1 Rabbit Polyclonal Antibody

SKU: orb185596

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Description

Research Area

DNA and RNA Research, RNA Processing

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Equine, Porcine, Rabbit, Rat, Sheep

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human METTL1 (1-100/276aa)
Target	METTL1
Molecular Weight	31 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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C12orf1; D1075 like gene product; FLJ95748; Methyltransferase like 1; Methyltransferase like protein 1; Methyltransferase-like protein 1; Mettl1; TRM8; TRMB_HUMAN; TRMT8; tRNA(guanine N(7) ) methyltransferase; tRNA(guanine(46)-N(7))-methyltransferase; tRNA(guanine-N(7)-)-methyltransferase; tRNA(m7G46) methyltransferase; tRNA(m7G46)-methyltransferase; YDL201w.

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Paraformaldehyde-fixed, paraffin embedded (Mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (METTL1) Polyclonal Antibody, Unconjugated (orb185596) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-METTL1 (orb185596) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 31 kD, Observed band size: 32 kD.

Quick Database Links

Gene Symbol

METTL1

UniProt

Q9UBP6

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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