MI-3

SKU: orb1305224

Description

MI-3 is a small molecule inhibitor that disrupts the Menin-MLL protein interaction with an IC50 of 648 nM. It is a valuable research tool for studying the role of this interaction in leukemia, supporting both in vitro biochemical and cellular assays.

Research Area

Cell Biology, Epigenetics & Chromatin

Images & Validation

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Key Properties

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CAS Number	1271738-59-0
MW	375.55
Purity	98.66% (May vary between batches)
Formula	C₁₈H₂₅N₅S₂
SMILES	CC(C)c1cc2c(ncnc2s1)N1CCN(CC1)C1=NCC(C)(C)S1
Target	Histone Methyltransferase,Apoptosis,Epigenetic Reader Domain
Solubility	Ethanol:16 mg/mL (42.6 mM);DMSO:16 mg/mL (42.6 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);10% DMSO+40% PEG300+5% Tween-80+45% Saline:0.5 mg/mL (1.33 mM)

Bioactivity

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Target IC50	Menin-MLL:648 nM
In Vivo	In HEK293 cells, MI-3 was in close proximity to the protein target, which in turn produced a significant inhibitory effect on the menin-MLL-AF9 interaction. In human MLL leukemia cell lines carrying different MLL translocations, MI-3 effectively blocked cell differentiation and promoted cell apoptosis. Intracellularly, Amlodipine caused neuronal Ca2+ elevation by attenuating KCl depolarization. Read more...
Cell Research	The MLL-AF9 and E2A-HLF transduced murine BMC are plated in 12-well plates at the concentration of 5×103 cells/mL with 1 mL methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained with 100 μL iodonitrotetrazolium chloride at final concentration of 1 mg/mL, incubated at 37°C for 30 min and counted. To replate for the 2nd round, colonies are counted at day 6 without staining and cells were washed out by 1×PBS buffer and resuspended in IMDM medium containing 15% FBS, 1% penicillin/streptomycin and IL-3. 5×103 cells are plated in 12-well plates with 1ml methylcellulose medium M3234 containing 20% IMDM medium, 1% penicillin/streptomycin, IL-3 and 0.25% DMSO or compounds. 6 days later colonies are stained and counted. (Only for Reference) Read more...

Storage & Handling

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Storage	-20°C
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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HistoneMethyltransferase, Histone Methyltransferase, Inhibitor, Menin-MLL, Menin-MLL Inhibitor, Menin-MLL inhibitor 3, Epigenetic Reader Domain, EpigeneticReaderDomain, MI 3, MI3, MI-3, inhibit, Apoptosis

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Compound Identifiers

PubChem CID

51001299 ↗

Key Properties

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No computed properties available.

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MI-3 (orb1305224)

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MI-3

Description

Research Area

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Key Properties

Bioactivity

Storage & Handling

Alternative Names

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