MICA Antibody (Center)

SKU: orb1928388

Description

Images & Validation

−

Item 1 of 7

Tested Applications	FC, IF, IHC-P, WB
Dilution range	IHC-P - 1:100-500, WB - 1:2000
Reactivity	Human

Key Properties

−

Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Molecular Weight	42915 Da
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Disclaimer	For research use only

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

MICA Antibody (Center) western blot analysis in MDA-MB-231 cell line lysates (35 ug/lane).This demonstrates the MICA antibody detected the MICA protein (arrow).

Anti-MICA Antibody (Center) at 1:2000 dilution + A431 whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-MICA Antibody (Center) at 1:2000 dilution + U-87 MG whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MICA Antibody (Center) at 1:2000 dilution. Lane 1: U-87 MG whole cell lysates. Lane 2: A431 whole cell lysates. Lane 3: Jurkat whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-MICA Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: Daudi whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MCF-7 whole cell lysate. Lane 5: U-87 MG whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 43 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling MICA at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).

Quick Database Links

UniProt

Q29983

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

IHC-P

Immunohistochemistry Paraffin

Flow Cytometry

Immunofluorescence