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Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Infectious Disease & Virology, Metabolism Research, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF - 1:10-50, WB - 1:1000 |
| Reactivity | Human |
| Predicted Reactivity | Mouse, Porcine, Rabbit, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Clone No. | B355EV37X32X7X3 |
| Immunogen | This MMP14 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 470-499 amino acids from the C-terminal region of human MMP14. Antigen Region: 470-499 aa. |
| Target | MMP14 |
| Molecular Weight | 65894 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Matrix metalloproteinase-14, MMP-14, MMP-X1, Membrane-type matrix metalloproteinase 1, MT-MMP 1, MTMMP1, Membrane-type-1 matrix metalloproteinase, MT1-MMP, MT1MMP, MMP14
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Anti-MMP14 Antibody (C-term) at 1:1000 dilution + HT-1080 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 65 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of MMP14 Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_004986.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
MMP14 Antibody (C-term) (orb1939417)
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