mOrange Antibody

SKU: orb1463294

Description

Goat polyclonal antibody to mOrange (Orange fluorescent protein). mOrange is a basic (constitutively fluorescent), monomeric engineered derivate of red fluorescent protein (RFP) isolated from members of the Discosoma (mushroom coral) family. mOrange is a ~27 kDa protein that is optimally excited at a 548 nm and has a maximum of emission at 562 nm. It is used in research as a reporter to label and study the biology of the cell using a wide range of applications.

Images & Validation

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Tested Applications	IEM, IF, IHC-Fr, IHC-P, WB
Dilution Range	WB:1:500-1:5,000, IF:1:50-1:500, IHC-P:1:50-1:500, IHC-F:1:50-1:500, IEM:1:50-1:500
Application Notes	The antibody solution should be gently mixed before use.

Key Properties

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Antibody Type	Primary Antibody
Host	Goat
Clonality	Polyclonal
Isotype	IgG
Immunogen	Antigen: Purified recombinant fluorescent protein produced in E. coli.
Target	Red Fluorescent Protein
Purification	Epitope affinity purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS, 20% glycerol and 0.05% sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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red fluorescent protein, Orange antibody.

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Quick Database Links

Gene Symbol

Red Fluorescent Protein

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Datasheet

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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