Mouse Human Bence Jones lambda (surface and hidden determinants), conjugated with Biotin Antibody

SKU: orb21183

Description

Biotin-conjugated purified monoclonal mouse antibody to human Ig lambda chain, free and bound. To identify the light chain type of immunoglobulins or free light chains in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100; in ELISA from 1:100 upwards; in Western blotting from 1:200 upwards. These data should be interpreted as general recommendations only.

Images & Validation

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Reactivity	Human

Key Properties

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Antibody Type	Secondary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1
Clone No.	NI 412, NI 268
Purification	ProteinA/G affinity purification
Conjugation	Biotin

Storage & Handling

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Storage	The lyophilized product may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.
Form/Appearance	No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Disclaimer	For research use only

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