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Description
Research Area
Cancer Biology
Images & Validation
−| Tested Applications | FC, IHC, IP, WB |
|---|---|
| Dilution Range | WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:50 - 1:200. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | DO-1 |
| Immunogen | Recombinant full length protein corresponding to Human p53 (N terminal). Epitope is within aa 20-25 |
| Target | p53 |
| Purification | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 100 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−antigen NY-CO-13; BCC7; cellular tumor antigen p53; LFS1; mutant tumor protein 53; P53; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; TRP53; tumor protein 53; Tumor suppressor p53; tumor supressor p53
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Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000537.3 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IP
Immunoprecipitation



















![Anti-p53 [PAb421]](/images/pub/media/catalog/product/NewWebsite/35/orb411547_1.png)
![Anti-p53 [PAb421]](/images/pub/media/catalog/product/NewWebsite/35/orb411547_2.png)
![Anti-p53 [PAb421]](/images/pub/media/catalog/product/NewWebsite/35/orb411547_3.png)
![Anti-p53 [PAb421]](/images/pub/media/catalog/product/NewWebsite/35/orb411548_1.png)
![Anti-p53 [PAb421]](/images/pub/media/catalog/product/NewWebsite/35/orb411548_2.png)
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