Mouse C4B(Complement C4-B) Microsample ELISA Kit

SKU: orb2999180

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Metabolism Research

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C4B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C4B in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	3.13-200 µg/mL
Sensitivity	1.32 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CO4; CPAMD3; Basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3

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Mouse C4B(Complement C4-B) Microsample ELISA Kit

Quick Database Links

UniProt

P01029

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No UniProt data available

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