Mouse Calcium Sensing Receptor (CASR) ELISA Kit

SKU: orb780979

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Sensing Receptor(CASR).

Research Area

Metabolism Research, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CASR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CASR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CASR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CASR in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.058 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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CAR ELISA Kit, FHH ELISA Kit, FIH ELISA Kit, GPRC2A ELISA Kit, HHC ELISA Kit, HHC1 ELISA Kit, NSHPT ELISA Kit, PCAR1 ELISA Kit, Parathyroid cell calcium-sensing receptor 1 ELISA Kit, Hypocalciuric Hypercalcemia 1 ELISA Kit, Severe Neonatal Hyperparathyroidism ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

Q9QY96

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Mouse
Dynamic Range:	0.16-10ng/mL
Sensitivity:	0.1 ng/mL

Reactivity:	Mouse
Dynamic Range:	0.156-10ng/ml
Sensitivity:	0.094ng/ml

Reactivity:	Mouse
Dynamic Range:	0.16-10ng/mL
Sensitivity:	0.06ng/mL

Reactivity:	Mouse
Dynamic Range:	0.16-10ng/mL
Sensitivity:	0.09ng/mL

Reactivity:	Mouse
Dynamic Range:	0.156-10 ng/mL
Sensitivity:	0.057 ng/mL