Mouse CCR6(Chemokine C-C-Motif Receptor 6) Microsample ELISA Kit

SKU: orb2997641

Description

Research Area

Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CCR6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CCR6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CCR6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CCR6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.054 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD196; BN-1; CKR-L3; CKR6; CKRL3; CMKBR6; DCR2; DRY-6; GPR-CY4; GPR29; GPRCY4; STRL22; Chemokine receptor-like 3; LARC receptor; G-protein coupled receptor 29

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UniProt

O54689

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No UniProt data available

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