Mouse Cluster of Differentiation 8 (CD8) ELISA Kit

SKU: orb1807997

Featured

Description

Research Area

Gastroenterology & Hepatology, Metabolism Research, Musculoskeletal & Connective Tissue Research

Images & Validation

−

Item 1 of 1

Application Notes

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse CD8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CD8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse CD8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse CD8. You can calculate the concentration of Mouse CD8 in the samples by comparing the OD of the samples to the standard curve.

Key Properties

−

Reactivity	Mouse
Sample Types	Tissue homogenate and Cell lysate
Assay Type	Sandwich
Assay Time	3.5H
Range	1.25-80ng/mL
Sensitivity	0.75 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

CD8

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Mouse Cluster of Differentiation 8 (CD8) ELISA Kit

Quick Database Links

Gene Symbol

CD8

UniProt

P01731, P10300

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

Find More Protocols