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Mouse Csf1r Antibody (C-term)
SKU: orb1936300
Description
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC-P, WB |
|---|---|
| Dilution range | IF - 1:25, WB - 1:2000 |
| Reactivity | Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 109179 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Csfmr, Fms
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Mouse Csf1r Antibody (C-term) western blot analysis in mouse heart tissue lysates (35 ug/lane). This demonstrates the Csf1r antibody detected the Csf1r protein (arrow).
All lanes: Anti-Mouse Csf1r Antibody (C-term) at 1:1000 dilution. Lane 1: Mouse heart lysate. Lane 2: Mouse skeletal muscle lysate. Lane 3: Rat skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Anti-Mouse Csf1r Antibody (C-term) at 1:1000-2000 dilution. Lane 1: Mouse heart tissue lysate. Lane 2: Mouse skeletal muscle tissue lysate. Lane 3: Mouse spleen tissue lysate. Lane 4: Rat brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 109 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labeling Csf1r at 1/25 dilution, followed by Dylight 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Cytoplasm staining on NIH/3T3 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).
Staining Mouse Csf1r in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
Mouse Csf1r Antibody (C-term) (orb1936300)
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