Mouse HE4(Epididymal protein 4) Microsample ELISA Kit

SKU: orb2999487

Description

Research Area

Cancer Biology

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HE4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HE4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.071 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Epididymis Protein 4; dJ461P17.6 ; EDDM4 ; epididymal protein 4 ; Epididymal secretory protein E4 ; Epididymis specific whey acidic protein type four disulfide core ; HE 4 ; Major epididymis specific protein E4 ; Major epididymis-specific protein E4 ; MGC57529 ; Putative protease inhibitor WAP5 ; WAP 5 ; WAP domain containing protein HE4 ; WAP domain containing protein HE4 V4 ; WAP four disulfide core domain 2 ; WAP four disulfide core domain protein 2 ; WAP four-disulfide core domain protein 2 ; WAP5 ; WFDC 2 ; WFDC2 ; WFDC2_HUMAN

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UniProt

Q9DAU7

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