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Mouse Hypoxia Inducible Factor 2 Alpha (HIF-2α) ELISA Kit

SKU: orb1948033
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Description

Mouse Hypoxia Inducible Factor 2 Alpha (HIF-2α) ELISA Kit

Research Area

Cardiovascular Research, Gastroenterology & Hepatology, Metabolism Research

Images & Validation

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Application Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-2α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-2α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF-2α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HIF-2α. You can calculate the concentration of Mouse HIF-2α in the samples by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityMouse
Sample Typesserum, plasma, Tissue homogenate and Other biological samples
Assay TypeSandwich
Assay Time3.5H
Range0.32-20ng/mL
Sensitivity0.19 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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EPAS1, ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, endothelial PAS domain protein 1, Hypoxia-inducible factor-2alpha

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol
HIF-2α
UniProt
P97481

UniProt Details

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No UniProt data available

Protocol Information

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Available Sizes

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48 T
$ 310.00
96 T
$ 430.00
DispatchUsually dispatched within 5-10 working days
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Email

[email protected]

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Distributors

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