Mouse IgG F(ab')2 Antibody Biotin Conjugated

SKU: orb347590

Description

Mouse IgG F(ab')2 antibody (Biotin)

Images & Validation

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Item 1 of 1

Tested Applications	DOT, ELISA, IHC, WB
Dilution range	ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Reactivity	Mouse
Application Notes	Anti-Mouse IgG F(ab')2 Biotin antibody has been tested by ELISA and dot blot and is assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse IgG F(ab')2 fragment
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(ab’)2 and Mouse Serum. No reaction was observed against Mouse IgG F(c).
Conjugation	Biotin

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	2.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Mouse IgG F(ab')2 Antibody Biotin Conjugate, Rabbit Anti Mouse IgG Fab2 Biotin Conjugated Antibody, Rabbit Anti Mouse IgG Fab2 Fragment Antibody Biotin Conjugation

Mouse IgG F(ab')2 Biotin Antibody [orb346296]

Conjugation:	Biotin
Purity:	This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Source:	Mouse

Sizes Available: 1 mg

Mouse IgG F(ab')2 Antibody Biotin Conjugated Pre-Adsorbed [orb347597]
Applications:
ELISA, IHC, WB
Reactivity:
Mouse
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 1 mg

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Mouse IgG F(ab')2 Antibody Biotin Conjugated

ELISA results of purified Rabbit Anti-Mouse IgG F(ab')2 Biotin Conjugated Antibody tested against purified Mouse IgG F(ab')2. Each well was coated in duplicate with 1.0 µg of Mouse IgG F(ab')2 (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer, Streptavidin HRP conjugate, and TMB substrate.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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DOT

Dot Blot

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