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Mouse IgG2b Isotype Control APC Antibody

SKU: orb154458
This product can be custom made

Description

Mouse monoclonal antibody conjugated to APC which recognizes IgG2b that belongs to many immunoglobulin G. It can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells. It was found that IgG2a was superior to IgG1 in activating complement. IgG2 is an antibody moleculer induced by infection.

Images & Validation

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Tested ApplicationsFC
Application Notes
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.

Key Properties

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Antibody TypeSecondary Antibody
ClonalityMonoclonal
IsotypeMouse IgG2b kappa
Clone No.MPC-11
ImmunogenKLH-coupled trinitrophenol
PurificationPurified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationAPC

Storage & Handling

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StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration0.1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

NCBI Gene ID
35

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

FC
Flow Cytometry
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Available Sizes

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0.1 mg
$ 370.00
Free Secondary Antibody (20 ul)0/0

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