Mouse IgM Antibody Alkaline Phosphatase Conjugated

SKU: orb347562

Description

Mouse IgM antibody (Alkaline Phosphatase)

Images & Validation

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Tested Applications	ELISA
Dilution range	ELISA: 1:20,000
Reactivity	Mouse
Application Notes	Anti-MOUSE IgM (alpha chain) Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user. Read more...

Key Properties

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse IgM whole molecule
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgM, and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
Conjugation	AP

Storage & Handling

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.1% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL
Disclaimer	For research use only

Alternative Names

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Rabbit Anti-Mouse IgM (mu chain) Antibody alkaline phosphatase conjugated, Rabbit Anti-Mouse IgM mu chain Antibody alk phos Conjugation

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Mouse IgM Antibody Alkaline Phosphatase Conjugated

ELISA Results of Rabbit Anti-Mouse IgM Antibody Alkaline Phosphatase Conjugate tested against purified Mouse IgM Alk Phos. Each well was coated in duplicate with 1.0 µg of Mouse IgM (p/n orb346283). The working dilution is 1:6700. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer and NPP Working Buffer.

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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