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Mouse IL-1a
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Tag | Tag free |
| Molecular Weight | Predicted: 18 KDa. Observed: 16 KDa, reducing conditions |
| Expression Region | Ser115-Ser270 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Sucrose, 2%Mannitol, 50mM Arginine, 0.03% Tween80, pH 8.0. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Mouse IL1 alpha protein (Active) [orb359005]
> 97% as determined by SDS-PAGE and HPLC.
17.9 kDa
E.Coli
10 μg, 100 μg, 500 μgRecombinant mouse IL-1 Alpha protein, His (HEK293) [orb1516667]
>95% as determined by SDS-PAGE
24 kDa
100 μg, 500 μg, 20 μgHuman IL1A Protein, mFc Tag [orb2321373]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide. The apparent molecular mass of IL1A-mFc is approximately 35-70 kDa due to glycosylation.
Mammalian
100 μg, 50 μg, 10 μgMouse IL-1 alpha protein [orb753806]
ELISA, WB
Greater than 95% as determined by SDS-PAGE
17.1 kDa
E.Coli
1 mg, 200 μg, 100 μg, 50 μg

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Protocol Information
Mouse IL-1a (orb3002350)
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