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Mouse Lipopolysaccharide (LPS) ELISA Kit

SKU: orb776857
Free Kit Promotion

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipopolysaccharide(LPS).

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA
Application Notes
standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LPS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LPS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LPS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LPS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityMouse
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.16-10 ng/mL
Sensitivity0.051 ng/mL
Concentration10 ng/mL

Procedure & Performance

Assay Principle
The kit is based on a sandwich enzyme immunoassay principle. The microtiter plate is pre-coated with a capture antibody specific to the target analyte. Standards or samples are added to the wells, followed by a biotin-conjugated detection antibody specific for the analyte. Avidin conjugated to horseradish peroxidase (HRP) is then added and incubated. After addition of the TMB substrate, color develops only in wells containing the analyte bound to the detection antibody and HRP–avidin complex. The reaction is stopped with an acidic solution, and absorbance is measured at 450 nm ± 10 nm. The analyte concentration in the samples is determined by comparison with a standard curve.
Kit Components
1. ELISA Microplate
2. Standards
3. Detection Antibody
4. HRP-Streptavidin Conjugate
5. TMB Substrate
6. Dilution buffers
7. Stop Solution
8. Wash Buffer
9. Plate Sealers
10. Manual
Reagent Preparation
1. Wash Buffer: Prepare the 1X Wash Buffer using distilled water according to the manual.
2. Standard: Perform gradient dilution according to the instructions in the manual.
3. Other Concentrated Reagents: Dilute the concentrated reagents using the Dilution Buffers provided in the kit to 1 X working solutions as instructed in the manual. Always use a clean pipette tip for each different solution.
Assay Procedure
This procedure is for reference only.

1. After the kit equilibrates to room temperature, add standards or samples to each well and incubate.
2. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper.
3. Add biotinylated antibody working solution to each well and incubate.
4. Discard liquid, add wash buffer to each well, wash the plate three times, and blot dry on clean absorbent paper.
5. Add streptavidin-HRP working solution to each well and incubate._x000b_6. Discard liquid, add wash buffer to each well, wash the plate five times, and blot dry on clean absorbent paper.
7. Add TMB substrate solution to each well and incubate in the dark.
8. Add stop solution to each well, mix thoroughly, and immediately read OD at 450 nm.
Materials Required
1. Microplate readers
2. Centrifuge
3. Incubator
4. Automated plate washer
5. Single-channel or multi-channel high-precision pipettes
6. Disposable pipette tips
7. Sterile tubes
8. Eppendorf tubes
9. Absorbent paper
10. Loading slots
Precision
Intra-assay Precision (Precision within an assay): CV% < 8%
Intra-assay precision was evaluated by testing multiple replicates of samples within the same plate.

Inter-assay Precision (precision between assays): CV% < 10%
Inter-assay precision was evaluated by testing samples across different plates.
Calculation of Results
1. Average the duplicate readings for each Standard, Control, and Sample, and subtract the mean optical density of the zero Standard.
2. Construct a standard curve by plotting the target concentration on the y-axis against absorbance on the x-axis and draw a curve through the data points.
3. Determine the sample concentration by substituting the OD450 value into the standard curve. For diluted samples, multiply the calculated value by the corresponding dilution factor.
Curve Fitting Softwares
1. Curve Expert
2. Thermo SkanIt RE
3. SciDAVis
4. LabPlot
5. ……

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

LOS ELISA Kit, Lipoglycans ELISA Kit, Lipooligosaccharide ELISA Kit, Lipo-Oligosaccharide ELISA Kit, Endotoxin ELISA Kit

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Mouse Lipopolysaccharide (LPS) ELISA Kit

Standard curve for orb776857 ELISA kit.

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Datasheet
Product Information
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Manual
Product Manual
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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Mouse Lipopolysaccharide (LPS) ELISA Kit (orb776857)

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Applications: ELISA|Reactivity: Mouse

该试剂盒采用成熟稳定的夹心ELISA检测体系,具有较高的特异性、重复性和检测稳定性,适用于小鼠样本中LPS水平的定量分析。相较于微量样本版本,其检测灵敏度更高、低浓度样本检测能力更强,尤其适用于需要准确评估微小浓度变化的实验研究。此外,其样本适用范围广、操作流程规范,能够满足基础研究及相关机制研究中对LPS检测的需求。

- 蔡思敏上海药物研究所
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Applications: ELISA|Reactivity: Mouse

该试剂盒采用双抗体夹心ELISA法检测小鼠样本中的脂多糖(LPS)水平,具有较高的检测灵敏度,最低检测限可达 0.051 ng/mL,能够满足低丰度样本的检测需求。试剂盒检测范围为 0.16–10 ng/mL,线性范围适中,可覆盖多数生物样本中LPS的浓度变化区间。同时,该试剂盒适用于血清、血浆、组织匀浆、细胞裂解液及细胞培养上清等多种样本类型,具有较好的适用性和兼容性。

- Yuxin Bi首都医科大学 / 心律失常
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Applications: ELISA|Reactivity: Mouse

This kit is primarily used to detect IL-6 expression levels in mouse samples and is well suited for sample types such as cell culture supernatants. During actual use, the operating procedures are clear, the reagents are complete, the standard curve shows good linearity, and the variation between duplicate wells is small. The assay results effectively reflect the changes in IL-6 levels among different treatment groups, providing good reference value for evaluating inflammatory responses and alterations in SASP-related factors. Overall, the user experience is positive, and this kit is recommended for ELISA applications.

- ZhuJianguoNanjing Agricultural University / animal

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48 T
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