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Description
Research Area
Cardiovascular Research, Neuroscience, Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB: 1:2000, IHC: 1:25 |
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | This mouse Nkx2-5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 98-133 amino acids from the Central region of mouse Nkx2-5. Antigen Region: 98-133 aa. |
| Target | Nkx2-5 |
| Molecular Weight | 34163 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Homeobox protein Nkx-25, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E, Nkx2-5, Csx, Nkx-25, Nkx2e
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All lanes: Anti-Nkx2-5 Antibody (Center) at 1:2000 dilution. Lane 1: mouse spleen lysates. Lane 2: mouse stomach lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 34 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Staining Mouse Nkx2-5 in mouse spleen sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Quick Database Links
Gene Symbol
Nkx2-5
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
Mouse Nkx2-5 Antibody (Center) (orb1787994)
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