Mouse Nuclear Factor Kappa B2 (NFkB2) ELISA Kit

SKU: orb776073

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B2(NFkB2).

Research Area

Signal Transduction

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NFkB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NFkB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NFkB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NFkB2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Mouse
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.059 ng/mL
Concentration	10 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

−

LYT-10 ELISA Kit, H2TF1 ELISA Kit, Lymphocyte translocation chromosome 10 ELISA Kit, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2(p49/P100) ELISA Kit, Nuclear factor NF-kappa-B p52 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Mouse Nuclear Factor Kappa B2 (NFkB2) ELISA Kit

Quick Database Links

UniProt

Q9WTK5

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols