Mouse S100A10(S100 Calcium Binding Protein A10) Microsample ELISA Kit

SKU: orb2999045

Description

Images & Validation

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Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100A10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100A10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100A10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100A10 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Sample Types	Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	12.4 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ANX2L; ANX2LG; CAL1L; CLP11; GP11; P11; P10; Calpactin-1 light chain; Cellular ligand of annexin II; Annexin II Ligand,Calpactin I,Light Polypeptide

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UniProt

P08207

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