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(Mouse) Smad1 Antibody (Center)
SKU: orb1926558
Description
Research Area
Stem Cell & Developmental Biology
Images & Validation
−Item 1 of 2
| Tested Applications | IHC-P, WB |
|---|---|
| Dilution range | IHC-P - 1:100-500, WB - 1:1000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Molecular Weight | 52157 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Disclaimer | For research use only |
Alternative Names
−Madh1, Madr1
Quality Guarantee
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Staining (Mouse) Smad1 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Anti-Smad1 Antibody (Center) at 1:1000 dilution + mouse skeletal muscle lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
(Mouse) Smad1 Antibody (Center) (orb1926558)
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