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Recombinant MPO Antibody / Myeloperoxidase

SKU: orb2636658

Description

Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

Images & Validation

Tested ApplicationsIHC-P, WB
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
ReactivityHuman
Application Notes
Optimal dilution of the recombinant MPO antibody should be determined by the researcher.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityRecombinant
IsotypeMouse IgG1, kappa
Clone No.rMPO/6904
ImmunogenA portion of amino acids 150-250 was used as the immunogen for the recombinant MPO antibody.
PurificationProtein A/G affinity
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
DisclaimerFor research use only

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Recombinant MPO Antibody / Myeloperoxidase

SDS-PAGE analysis of purified, BSA-free MPO antibody (clone rMPO/6904) as confirmation of integrity and purity.

Recombinant MPO Antibody / Myeloperoxidase

IHC staining of FFPE human bone marrow with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Recombinant MPO Antibody / Myeloperoxidase

IHC staining of FFPE human tonsil tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Recombinant MPO Antibody / Myeloperoxidase

IHC staining of FFPE human spleen tissue with recombinant MPO antibody (clone rMPO/6904). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Recombinant MPO Antibody / Myeloperoxidase

Western blot testing of human spleen tissue lysate using recombinant MPO antibody (clone rMPO/6904). Expected molecular weight: 75-90 kDa (pro form).

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol

Recombinant MPO Antibody / Myeloperoxidase (orb2636658)

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100 μg
¥ 9,750.00
DispatchUsually dispatched within 5-10 working days
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