TMEM173 Antibody

SKU: orb1239632

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Description

TMEM173 Antibody

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 6

Tested Applications	ELISA, ICC, IF, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Gallus, Porcine, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-MPYS antibody (orb1239632) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human MPYS. The immunogen is located within amino acids 260-310 of MPYS.
Target	TMEM173
Molecular Weight	Predicted: 42kDObserved: 42 kD
Purification	MPYS Antibody is affinity chromatography purified via peptide column.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	MPYS Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MPYS Antibody: ERIS, MITA, MPYS, NET23, STING, ERIS, Stimulator of interferon genes protein, Endoplasmic reticulum interferon stimulator, hSTING

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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (1 µg/mL), MPYS orb1271001 (2 µg/mL) and beta-actin orb1240312 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human THP-1 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse A-20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: MPYS orb1239632 (Lane 1: 1 µg/mL and Lane 2: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry Validation of MPYS in Mouse A20 Cells. Immunocytochemical analysis of A20 cells using anti-MPYS antibody (orb1239632) at 5 µg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of MPYS in Moue A20 Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling MPYS with orb1239632 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Quick Database Links

Gene Symbol

TMEM173

UniProt

Q86WV6

RefSeq (Protein):NP_938023

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_938023

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ICC

Immunocytochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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