MUC1 Rabbit Polyclonal Antibody

SKU: orb1735096

Description

Research Area

Cancer Biology

Images & Validation

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Item 1 of 6

Tested Applications	IF, IHC, WB
Dilution Range	Western Blot 1:500 - 1:1000, Immunofluorescence 1:100 – 1:400, Immunohistochemistry (Paraffin) 1:100
Reactivity	Human
Application Notes	Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit-IgG
Immunogen	Synthetic peptide/MUC1 extracellular domain
Target	MUC1 / Mucin 1 / CD227
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: MUC1 antibody (extracellular) (orb1735096).

Immunohistochemical analysis of paraffin embedded Human colorectal adenocarcinoma and muscle tissue labeling MUC1 antibody with orb1735096 at 1/100.

Immunohistochemical analysis of paraffin embedded Human lung Carcinoma tissue labeling MUC1 antibody with orb1735096 at 1/100.

Immunohistochemical analysis of paraffin embedded Human muscle and placenta tissue labeling MUC1 antibody with orb1735096 at 1/100.

Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling MUC1 antibody with orb1735096 at 1/100.

This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated overnight at 4°C. Lysates/proteins at 35 µg per lane. Anti-MUC1 antibody - at 1/700 dilution.

Quick Database Links

Gene Symbol

MUC1 / Mucin 1 / CD227

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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