Mucin 1 (phospho Tyr1229) rabbit pAb

SKU: orb769163

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Description

Rabbit polyclonal antibody to Mucin 1 (phospho-Tyr1229)

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IF, IHC-P, WB
Dilution range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human CD227/MUC1 around the phosphorylation site of Tyr1229. AA range:1201-1250
Molecular Weight	170
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Anti-MUC1 antibody, anti-PUM antibody, anti-Mucin-1 antibody, anti-MUC-1 antibody, anti-Breast carcinoma-associated antigen DF3 antibody, anti-Carcinoma-associated mucin antibody, anti-Episialin antibody, anti-H23AG antibody, anti-Krebs von den Lungen-6 antibody, anti-KL-6 antibody, anti-PEMT antibody, anti-Peanut-reactive urinary mucin antibody, anti-PUM antibody, anti-Polymorphic ep antibody

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Immunofluorescence analysis of HepG2 cells, using CD227/MUC1 (Phospho-Tyr1229) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CD227/MUC1 (Phospho-Tyr1229) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30', using CD227/MUC1 (Phospho-Tyr1229) Antibody. The lane on the right is blocked with the phospho peptide.

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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