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N4BP1 Antibody
SKU: orb1239410
Description
Research Area
Cancer Biology, Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, ICC, IF, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.The immunogen is located within the first 50 amino acids of N4BP1. |
| Target | N4BP1 |
| Purification | N4BP1 Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Disclaimer | For research use only |
Alternative Names
−N4BP1 Antibody: KIAA0615, NEDD4-binding protein 1, N4BP1
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WB Validation in Human Cell LinesLoading: 15 µg of lysates Antibodies: N4BP1, orb1239410 1 µg/ml, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Exposure: 30 sec.
Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 µg/mL.
Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 µg/mL.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
N4BP1 Antibody (orb1239410)
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