Recombinant CD56 Antibody

SKU: orb1825998

Description

This mAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Images & Validation

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Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 2-4ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant CD56 antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	NCAM1/8392R
Immunogen	A recombinant partial protein (within amino acids 600-800) from the human protein was used as the immunogen for the recombinant CD56 antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human colon tissue with recombinant CD56 antibody (clone NCAM1/8392R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD56 antibody (clone NCAM1/8392R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P13592, P13591

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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