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NDRG2 Recombinant Mouse Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ICC, WB |
|---|---|
| Dilution range | WB=1:500-2000, ICC/IF=1:100-500 |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Recombinant |
| Isotype | IgG2b/Kappa |
| Clone No. | F8A5 |
| Target | NDRG2 |
| Purification | Affinity purified by Protein G |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Disclaimer | For research use only |

Quality Guarantee
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Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:2000, Primary ab incubation condition: room temperature 2h, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: Mouse brain, Rat heart, Protein loading quantity: 20 µg, Exposure time: 3 s, Predicted MW: 41 kDa, Observed MW: 41 kDa.

Cell line: HepG2, Fixative: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary ab Dilution: 1:50, Primary incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1172545.
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Protocol Information
NDRG2 Recombinant Mouse Monoclonal Antibody (orb1172545)
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