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Description
Research Area
Cell Biology, Complex I, Energy Metabolism, Metabolism, Mitochondrial, Mitochondrial Markers, Oxidative Stress, Respiratory Disease
Images & Validation
−Item 1 of 1
| Tested Applications | IF, IHC-Fr, IHC-P |
|---|---|
| Dilution Range | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Reactivity | Mouse |
| Predicted Reactivity | Bovine, Canine, Equine, Human, Porcine, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human NDUFA8 (101-172/172aa) |
| Target | NDUFA8 |
| Molecular Weight | 19 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Complex I 19kD; CI 19KD; CI PGIV; Complex I 19k; Complex I PGIV; MGC793; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8(19kD PGIV); NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8 19kDa; NADH dehydrogenase(ubiquinone) 1 alpha subcomplex 8; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8; NADH ubiquinone oxidoreductase 19 kDa subunit; NADH ubiquinone oxidoreductase PGIV subunit; NDUFA 8; PGIV.
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Tissue/Cell: mouse brain tissue 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-NDUFA8 Polyclonal Antibody, Unconjugated (orb6129) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Quick Database Links
Gene Symbol
NDUFA8
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
IF
Immunofluorescence
NDUFA8 Rabbit Polyclonal Antibody (orb6129)
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