Recombinant NeuN Antibody / Fox3

SKU: orb1151476

Description

Rabbit monoclonal antibody to NeuN

Research Area

Epigenetics

Images & Validation

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Item 1 of 4

Tested Applications	IHC-P, WB
Dilution range	Immunohistochemistry (FFPE): 1-2ug/ml,Western blot:1-2ug/ml
Reactivity	Human
Application Notes	Optimal dilution of the recombinant NeuN antibody should be determined by the researcher.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Recombinant
Isotype	Rabbit IgG, kappa
Clone No.	NeuN/288R
Immunogen	Recombinant full-length human RNA binding protein fox-1 homolog 3 protein was used as the immunogen for the recombinant NeuN antibody.
Purification	Protein A/G affinity
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

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IHC staining of FFPE human cerebellum tissue with recombinant NeuN antibody (clone NeuN/288R). Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebrum tissue with recombinant NeuN antibody (clone NeuN/288R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human brain tissue lysate using recombinant NeuN antibody (clone NeuN/288R).

Western blot testing of human HeLa cell lysate using recombinant NeuN antibody (clone NeuN/288R).

Quick Database Links

UniProt

A6NFN3

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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