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Neurofilament L Rabbit Polyclonal Antibody
SKU: orb1294399
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | Western Blot 1:1000-1: 2000, Immunofluorescence 1:200-1:500 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Recombinant fragment corresponding to Human Neurofilament L. Encompassing a sequence within the C-terminus region. |
| Target | Neurofilament L / NF L |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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All lanes: anti-Neurofilament-L / NF-L antibody at 1/1000 dilution, Lysates/proteins at 60 µg per lane. This blot was produced using a 7.5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked an hour before being incubated with orb1294399 overnight at 4°C.
Immunofluorescent analysis. Sample: primary cortical neurons, NF-L antibody (orb1294399): 1-250, Red: Tuj1 antibody, Anti-rabbit 488: 1-500, Fixed: 4% paraformaldehyde at RT for 20 min.
Quick Database Links
Gene Symbol
Neurofilament L / NF L
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
Neurofilament L Rabbit Polyclonal Antibody (orb1294399)
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