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NFE2L2 Rabbit Polyclonal Antibody
SKU: orb1294327
Featured
Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 14
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000, Immunofluorescence 1:100-1:200, Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the center region |
| Target | NFE2L2 / NRF2 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Anti-NRF2 antibody at 1/800 dilution. Lysates at 30 µg per lane. This blot was produced using a 5% SDS-PAGE. NC membrane was then blocked for an hour before being incubated with orb1294327 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294327 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294327 anti-NRF2 antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling NRF2 with orb1294327 at 1/150.
Immunohistochemical analysis of paraffin embedded mouse Kidney tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse liver labeling NRF2 with orb1294327 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse lung labeling NRF2 with orb1294327 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling NRF2 with orb1294327 at 1/100 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Quick Database Links
Gene Symbol
NFE2L2 / NRF2
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
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Mina Kiani 1, Malek Soleimani Mehranjani 2, Mohammad Ali Shariatzadeh Empagliflozin reduces the adverse effects of diabetes mellitus on testicular tissue in type 2 diabetic Rats: A stereological and biochemical study Biochem Pharmacol ., (2024)
Applications
IHC
Reactivity
Rat
NFE2L2 Rabbit Polyclonal Antibody (orb1294327)
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