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NFkB p65 Monoclonal Antibody(5G6)
SKU: orb1415915
Description
Research Area
Epigenetics, Infectious Diseases, NF-kB, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC-P, IP, WB |
|---|---|
| Dilution range | WB: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300, IHC: 1:50-300, IF: 1:200, WB: 1:500-2000, IP: 1:200 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Disclaimer | For research use only |
Alternative Names
−RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
1) Input: Hela Cell Lysate 2) IP product: IP dilute 1: 200
Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Mouse Spleen Tissue, diluted at 1: 2000.
Immunofluorescence analysis of Human-appendix tissue. 1, NFkB p65 Monoclonal Antibody (5G6) (red) was diluted at 1: 200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1: 300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, NFkB p65 Monoclonal Antibody (5G6) was diluted at 1: 200 (4°C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1: 200 (room tempeRature, 30 min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Appendix tissue. 1, NFkB p65 Monoclonal Antibody (5G6) was diluted at 1: 200 (4°C, overnight). 2, Sodium citrate pH6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1: 200 (room tempeRature, 30 min). Negative control was used by secondary antibody only.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
IP
Immunoprecipitation
NFkB p65 Monoclonal Antibody(5G6) (orb1415915)
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